1KVE
KILLER TOXIN FROM HALOTOLERANT YEAST
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-20 |
| Detector | MACSCIENCE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.120, 81.120, 118.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.172 * |
| Rwork | 0.172 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.062 |
| Total number of observations | 185448 * |
| Number of reflections | 35468 |
| Completeness [%] | 94.7 |
| Redundancy | 5.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 | CRYSTALS WERE GROWN FROM 25% POLYETHYLENE GLYCOL SOLUTION AT PH 4.0. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 10 | 1 | reservoir | ammonium sulfate | 167 (mM) | |
| 11 | 1 | reservoir | glycine-HCl | 167 (mM) | |
| 2 | 1 | drop | PEG4000 | 15 (%(w/v)) | |
| 3 | 1 | drop | ammonium sulfate | 100 (mM) | |
| 4 | 1 | drop | glycine-HCl | 100 (mM) | |
| 5 | 1 | drop | citrate-PO4 | 8 (mM) | |
| 6 | 1 | drop | 70 (mM) | ||
| 7 | 1 | drop | EDTA | 0.5 (mM) | |
| 8 | 1 | drop | PMSF | 0.05 (M) | |
| 9 | 1 | reservoir | PEG4000 | 25 (%(w/v)) |
