1KVD
KILLER TOXIN FROM HALOTOLERANT YEAST
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1995-03-10 |
| Detector | WEISSENBERG |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.100, 81.100, 118.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| Rwork | 0.186 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.800 | |
| Rmerge | 0.069 | |
| Total number of observations | 168550 * | |
| Number of reflections | 35030 | |
| Completeness [%] | 93.7 | 82.7 * |
| Redundancy | 7.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 3.5 | 20 * | CRYSTALS WERE GROWN FROM 2.7M AMMONIUM SULFATE SOLUTION AT PH 3.5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.5 (M) | |
| 3 | 1 | drop | glycine-HCl | 50 (mM) | |
| 4 | 1 | drop | citrate-PO4 | 8 (mM) | |
| 5 | 1 | drop | 70 (mM) | ||
| 6 | 1 | drop | EDTA | 0.5 (mM) | |
| 7 | 1 | drop | PMSF | 0.05 (M) | |
| 8 | 1 | reservoir | ammonium sulfate | 2.7 (M) | |
| 9 | 1 | reservoir | glycine-HCl | 90 (mM) |
