1KUQ

CRYSTAL STRUCTURE OF T3C MUTANT S15 RIBOSOMAL PROTEIN IN COMPLEX WITH 16S RRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-21
Detector	MARRESEARCH
Wavelength(s)	0.933
Spacegroup name	P 64 2 2
Unit cell lengths	128.229, 128.229, 64.951
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.410 - 2.840
R-factor	0.231
Rwork	0.231
R-free	0.32200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dk1
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.920
High resolution limit [Å]	2.840	2.840
Rmerge	0.049	0.120
Number of reflections	7828
Completeness [%]	99.8	99.9
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	295	ammonium sulphate, KCl, MgCl2, sodium cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	ammonium sulphate
2	1	1	KCl
3	1	1	MgCl2
4	1	1	sodium cacodylate