1KTQ

DNA POLYMERASE

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Spacegroup name	P 21 21 2
Unit cell lengths	109.400, 136.800, 45.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.198
Rwork	0.198
R-free	0.26200
RMSD bond length	0.011
RMSD bond angle	22.320 *
Data reduction software	R-AXIS
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500
Rmerge	0.070
Total number of observations	66183 *
Number of reflections	22333 *
Completeness [%]	90.6
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	6 (%(w/v))
2	1	reservoir		50 (mM)
3	1	reservoir	Tris-HCl	100 (mM)