1KT7

Crystal structure of bovine holo-RBP at pH 7.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-10
Detector	MARRESEARCH
Wavelength(s)	0.9393
Spacegroup name	P 21 21 21
Unit cell lengths	45.907, 48.660, 75.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.866 - 1.274
R-factor	0.1345
Rwork	0.132
R-free	0.19090 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hbp
RMSD bond length	0.012
RMSD bond angle	2.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.540 *	1.340
High resolution limit [Å]	1.270 *	1.270 *
Rmerge	0.057	0.237
Total number of observations	226144 *
Number of reflections	42476 *
<I/σ(I)>	5.6	2
Completeness [%]	94.3	67.1
Redundancy	3.1	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	20 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium cacodylate	30 (mM)	pH7.
2	1	reservoir	sodium acetate	20 (mM)
3	1	reservoir	MPD	8 (%(v/v))
4	1	reservoir		0.5 (mM)