1KT3
Crystal structure of bovine holo-RBP at pH 2.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.839, 50.198, 77.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.246 - 1.400 |
| R-factor | 0.1753 |
| Rwork | 0.175 |
| R-free | 0.24350 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hbp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 * | 1.470 |
| High resolution limit [Å] | 1.390 * | 1.390 * |
| Rmerge | 0.069 | 0.382 * |
| Total number of observations | 334447 * | |
| Number of reflections | 32419 | |
| <I/σ(I)> | 5.7 | 1 |
| Completeness [%] | 87.8 | 42.7 |
| Redundancy | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 20 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium cacodylate | 30 (mM) | pH7. |
| 2 | 1 | reservoir | sodium acetate | 20 (mM) | |
| 3 | 1 | reservoir | MPD | 8 (%(v/v)) | |
| 4 | 1 | reservoir | 0.5 (mM) |
