1KT3

Crystal structure of bovine holo-RBP at pH 2.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-10
Detector	MARRESEARCH
Wavelength(s)	0.9393
Spacegroup name	P 21 21 21
Unit cell lengths	46.839, 50.198, 77.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.246 - 1.400
R-factor	0.1753
Rwork	0.175
R-free	0.24350 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hbp
RMSD bond length	0.015
RMSD bond angle	3.200 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.300 *	1.470
High resolution limit [Å]	1.390 *	1.390 *
Rmerge	0.069	0.382 *
Total number of observations	334447 *
Number of reflections	32419
<I/σ(I)>	5.7	1
Completeness [%]	87.8	42.7
Redundancy	3.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	20 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium cacodylate	30 (mM)	pH7.
2	1	reservoir	sodium acetate	20 (mM)
3	1	reservoir	MPD	8 (%(v/v))
4	1	reservoir		0.5 (mM)