1KT0

Structure of the Large FKBP-like Protein, FKBP51, Involved in Steroid Receptor Complexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	200
Detector technology	CCD
Collection date	1999-05-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9134
Spacegroup name	P 32 2 1
Unit cell lengths	87.583, 87.583, 132.033
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 * - 2.700
Rwork	0.284
R-free	0.37500
Structure solution method	MAD
RMSD bond length	0.010 *
RMSD bond angle	1.580 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.066 *
Total number of observations	80664 *
Number of reflections	16517 *
<I/σ(I)>	3.99	2.2
Completeness [%]	100.0	100
Redundancy	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	277	PEGMME 5000, ammonium sulfate, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH8.0
3	1	drop		50 (mM)
4	1	drop	EDTA	0.1 (mM)
5	1	drop	dithiothreitol	10 (mM)
6	1	reservoir	PEG5000 MME	15-18 (%)
7	1	reservoir	MES	100 (mM)	pH5.8
8	1	reservoir	ammonium sulfate	0.2 (M)