1KT0
Structure of the Large FKBP-like Protein, FKBP51, Involved in Steroid Receptor Complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 1999-05-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9134 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.583, 87.583, 132.033 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 * - 2.700 |
| Rwork | 0.284 |
| R-free | 0.37500 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.580 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.066 * | |
| Total number of observations | 80664 * | |
| Number of reflections | 16517 * | |
| <I/σ(I)> | 3.99 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 277 | PEGMME 5000, ammonium sulfate, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 10 (mM) | |
| 6 | 1 | reservoir | PEG5000 MME | 15-18 (%) | |
| 7 | 1 | reservoir | MES | 100 (mM) | pH5.8 |
| 8 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
