1KSY
Crystal Structures of Two Intermediates in the Assembly of the Papillomavirus Replication Initiation Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-14 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.111, 110.600, 75.084 |
| Unit cell angles | 90.00, 116.85, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 3.050 |
| R-factor | 0.2805 * |
| Rwork | 0.277 |
| R-free | 0.31720 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f08 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.930 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.108 | 0.478 |
| Total number of observations | 72557 * | |
| Number of reflections | 20851 | 2091 * |
| <I/σ(I)> | 10.2 | 1.6 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 3.5 | 3.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | MgSO4, spermine tetrahydrochloride, ethylene glycol, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgSO4 | ||
| 2 | 1 | 1 | spermine tetrahydrochloride | ||
| 3 | 1 | 1 | ethylene glycol | ||
| 4 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 20 (mM) | ||
| 2 | 1 | reservoir | 2.5 (mM) | ||
| 3 | 1 | reservoir | 5 (%) |
