1KSX
Crystal Structures of Two Intermediates in the Assembly of the Papillomavirus Replication Initiation Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-04 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.246, 103.650, 124.996 |
| Unit cell angles | 90.00, 99.53, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 3.200 |
| R-factor | 0.2634 * |
| Rwork | 0.263 |
| R-free | 0.28440 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f08 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 21.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.195 | 0.584 |
| Total number of observations | 125960 * | |
| Number of reflections | 35038 | 3474 * |
| <I/σ(I)> | 9.14 | 2.4 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | Ca(CF3COO)2, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ca(CF3COO)2 | ||
| 2 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 20 (mM) | ||
| 2 | 1 | reservoir | spermine tetrahydrochloride | 2.5 (mM) | |
| 3 | 1 | reservoir | ethylene glycol | 5 (%) |
