1KSK
STRUCTURE OF RSUA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.968634 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 48.771, 149.220, 35.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.000 |
| R-factor | 0.217 * |
| Rwork | 0.217 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.400 * |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 * | |
| Total number of observations | 103836 * | |
| Number of reflections | 31168 | |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 92.8 | 61.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 291 | PEG 3350, Malate, KSCN, Isoproponal, Glycerol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 14-16 (%(w/v)) | |
| 2 | 1 | reservoir | malate | 100 (mM) | pH5.6 |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | isopropanol | 18 (%) | |
| 5 | 1 | reservoir | glycerol | 2-5 (%(w/v)) | |
| 6 | 1 | drop | protein | 17 (mg/ml) | |
| 7 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 8 | 1 | drop | 200 (mM) | ||
| 9 | 1 | drop | glycerol | 5 (%(w/v)) |
