1KS8

The structure of Endoglucanase from termite, Nasutitermes takasagoensis, at pH 2.5.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-06-01
Detector	MAC Science DIP-2030K
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	89.710, 83.830, 60.680
Unit cell angles	90.00, 95.60, 90.00

Refinement procedure

Resolution	30.000 - 1.400
Rwork	0.177
R-free	0.18500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tf4
RMSD bond length	0.004
RMSD bond angle	1.240 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.062	0.543
Total number of observations	775989 *
Number of reflections	85117
<I/σ(I)>	22	2.7
Completeness [%]	96.9 *	89.4 *
Redundancy	9.117

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5 *	291	Ammonium Sulfate, Sodium Citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 2.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	sodium citrate	0.1 (M)	pH4.5
3	1	drop	ammonium sulfate	0.8 (M)