1KRU
Galactoside Acetyltransferase in Complex with IPTG and Coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-01-07 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.100, 183.800, 121.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.172 * |
| Rwork | 0.172 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 27.600 * |
| Data reduction software | X-GEN |
| Data scaling software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.088 | 0.231 |
| Total number of observations | 65103 * | |
| Number of reflections | 17213 * | |
| Completeness [%] | 92.2 | 67.5 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 298 | Wang, X.G., (1999) Acta Crystallogr., D55, 1955. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | pH7.8 |
| 3 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 4 | 1 | drop | acetyl-CoA | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.3 (M) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.4 |
| 7 | 1 | reservoir | L(+)-tartaric acid | 0.17 (M) |
