1KQU

Human phospholipase A2 complexed with a substrate anologue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-01-04
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.54
Spacegroup name	P 61 2 2
Unit cell lengths	76.550, 76.550, 93.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.209
Rwork	0.209
R-free	0.24000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1pod
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.091	0.346
Number of reflections	9218
<I/σ(I)>	19.3	3.18
Completeness [%]	93.8	92.5
Redundancy	5.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	sodium Chloride, Calcium Chloride, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K