1KQM
SCALLOP MYOSIN S1-AMPPNP IN THE ACTIN-DETACHED CONFORMATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.087 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.396, 58.642, 148.873 |
| Unit cell angles | 81.64, 82.39, 87.43 |
Refinement procedure
| Resolution | 39.810 - 3.000 |
| R-factor | 0.2812 |
| Rwork | 0.277 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b7t |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 31019 | |
| <I/σ(I)> | 11.7 | 5.6 |
| Completeness [%] | 80.6 | 53.7 |
| Redundancy | 3.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | PEG 20000, magnesium chloride, ethylene glycol, Tris HCl, AMPPNP, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
