1KQF
FORMATE DEHYDROGENASE N FROM E. COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 203.000, 203.000, 203.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.600 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.19500 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 342711 | |
| Completeness [%] | 94.9 | 93.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 3 | 1 | drop | OG | 1 (%) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.5-8.2 |
| 5 | 1 | reservoir | PEG1500 | 6-12 (%(w/v)) | |
| 6 | 1 | reservoir | 100 (mM) | ||
| 7 | 1 | reservoir | 100 (mM) | ||
| 8 | 1 | reservoir | ethanol | 5 (%) |
