1KQ9
Human methionine aminopeptidase type II in complex with L-methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.069, 98.660, 101.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.900 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.20900 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1bn5 |
| RMSD bond length | 0.070 * |
| RMSD bond angle | 1.880 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.042 | 0.046 |
| Number of reflections | 33600 | |
| <I/σ(I)> | 23.24 | |
| Completeness [%] | 94.0 | 96.5 |
| Redundancy | 3.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 277 | 18-23% t-butanol in 70 mM sodium citrate buffer, pH 5.3 to 5.6, 3 mM DTT (DL-dithiothreitol), VAPOR DIFFUSION, SITTING DROP at 277K, pH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.4 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | protein | 25 (mg/ml) | |
| 5 | 1 | reservoir | tert-butanol | 18-23 (%) | |
| 6 | 1 | reservoir | sodium citrate | 70 (mM) | pH5.3-5.6 |
