1KQ5
C-terminal Domain of Cyclase Associated Protein with PRO 505 Replaced by SER (P505S)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-11-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.04 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 56.370, 86.610, 160.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.000 |
R-factor | 0.237 |
Rwork | 0.230 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4z |
RMSD bond length | 0.007 |
RMSD bond angle | 1.310 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 3.190 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.058 | 0.097 |
Number of reflections | 7755 | |
<I/σ(I)> | 34.6 | 12.2 |
Completeness [%] | 97.5 | 93.2 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, lithium sulfate, hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |