1KPR

The human non-classical major histocompatibility complex molecule HLA-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-11
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	178.700, 178.700, 88.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.235
R-free	0.28900
Structure solution method	rigid-body refinement of 1MHE
Starting model (for MR)	1mhe
RMSD bond length	0.028
RMSD bond angle	27.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.052	0.379
Total number of observations	299482 *
Number of reflections	38312
<I/σ(I)>	13.4	3.3
Completeness [%]	95.8	97.3
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	22 *	ammonium sulfate, PEG 400, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP at 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.2 (M)
2	1	reservoir	PEG400	2 (%)
3	1	reservoir	Tris	100 (mM)	pH8.0