1KP6

USTILAGO MAYDIS KILLER TOXIN KP6 ALPHA-SUBUNIT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1996-09
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 63 2 2
Unit cell lengths	48.300, 48.300, 124.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.164
Rwork	0.164
R-free	0.21100
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.309
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.077
Total number of observations	109467 *
Number of reflections	8260
<I/σ(I)>		2.15
Completeness [%]	93.9	90
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6		drop1-0.005 ml, drop2-0.001 ml *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop1	protein	10 (mg/ml)
2	1	drop2	ammonium sulfate	85 (%)
3	1	drop2	MES	10 (mM)
4	1	reservoir	ammonium sulfate	18-21 (%)
5	1	reservoir	MES	10 (mM)