1KP5

Cyclic Green Fluorescent Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 65 2 2
Unit cell lengths	142.447, 142.447, 183.809
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.600
Rwork	0.208
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1emm
RMSD bond length	0.006
RMSD bond angle	26.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.078	0.503
Total number of observations	917968 *
Number of reflections	34481 *
<I/σ(I)>	26	6
Completeness [%]	97.8	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	285	ammonium sulfate, glycine, pH 10, VAPOR DIFFUSION, HANGING DROP, temperature 285K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.1 (M)
2	1	reservoir	glycine	0.1 (M)	pH10.0