1KP4

CALCIUM-BOUND FORM OF PROKARYOTIC PHOSPHOLIPASE A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	292
Detector technology	IMAGE PLATE
Collection date	1999-03
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	38.280, 54.250, 30.650
Unit cell angles	90.00, 90.20, 90.00

Refinement procedure

Resolution	5.000 - 1.600
Rwork	0.150
Structure solution method	AB INITIO
Starting model (for MR)	1faz
RMSD bond length	0.008
RMSD bond angle	0.023
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.065	0.283
Number of reflections	12820
<I/σ(I)>	11.2	2.41
Completeness [%]	73.2	48
Redundancy	2.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	297	MPD, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K