1KP0
The Crystal Structure Analysis of Creatine Amidinohydrolase from Actinobacillus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2001-02-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 111.263, 113.624, 191.649 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1chm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.320 * |
Data reduction software | DPS |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.065 | 0.151 |
Total number of observations | 167670 * | |
Number of reflections | 33902 | |
<I/σ(I)> | 10.6 | 4.9 |
Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | PEG 6000, Na citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10.0 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 10 (%) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.0 |