1KOP
NEISSERIA GONORRHOEAE CARBONIC ANHYDRASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.230, 74.940, 62.380 |
| Unit cell angles | 90.00, 93.87, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.900 |
| R-factor | 0.208 * |
| Rwork | 0.206 |
| R-free | 0.28500 * |
| Structure solution method | MR |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.240 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.072 |
| Number of reflections | 28517 |
| Completeness [%] | 83.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 6% PEG 3,350; 50MM TRIS; 0.1 M LI2SO4;2.2 MM BME; 2.2 MM NAN3; 10% DMSO, pH 7.8 |
