1KOI
CRYSTAL STRUCTURE OF NITROPHORIN 4 FROM RHODNIUS PROLIXUS COMPLEXED WITH NITRIC OXIDE AT 1.08 A RESOLUTION
Replaces: 1IKHExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.149, 42.419, 52.942 |
| Unit cell angles | 90.00, 94.22, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.080 |
| R-factor | 0.115 |
| Rwork | 0.115 |
| R-free | 0.14700 |
| Starting model (for MR) | 1erx |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.100 |
| High resolution limit [Å] | 1.000 | 1.080 |
| Rmerge | 0.040 | 0.240 |
| Number of reflections | 62726 | |
| <I/σ(I)> | 38 | 15 |
| Completeness [%] | 95.0 | 70 |
| Redundancy | 5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | PEG 4000, Sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
