1KOB
TWITCHIN KINASE FRAGMENT (APLYSIA), AUTOREGULATED PROTEIN KINASE DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY |
| Synchrotron site | Photon Factory |
| Detector technology | IMAGE PLATE |
| Collection date | 1995 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.300, 87.800, 152.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| R-factor | 0.1999 |
| Rwork | 0.200 |
| R-free | 0.28200 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.677 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.118 |
| Total number of observations | 153335 * |
| Number of reflections | 33532 |
| Completeness [%] | 88.1 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.4 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | ||
| 3 | 1 | reservoir | PEG4000 | 15 (%) | |
| 4 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 1 (mM) |
