1KO6
Crystal Structure of C-terminal Autoproteolytic Domain of Nucleoporin Nup98
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2000-04-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.900, 89.900, 203.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| Rwork | 0.247 |
| R-free | 0.27100 |
| Structure solution method | Molecular Replacement+MIR |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 41000 | |
| Completeness [%] | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.25 * | 300 | MgCl, PEG8000, Tris, pH 8.75, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 4 (mM) | |
| 2 | 1 | drop | 25 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 17 (%) | |
| 5 | 1 | reservoir | Tris | 0.1 (M) | pH8.25 |
| 6 | 1 | reservoir | 0.1 (M) | ||
| 7 | 1 | reservoir | dithiothreitol | 30 (mM) |
