1KO4
Crystal structure of gluconate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97830 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.231, 72.602, 79.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.060 - 2.600 * |
| R-factor | 0.24 * |
| Rwork | 0.240 |
| R-free | 0.30400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.580 |
| High resolution limit [Å] | 2.600 | 2.550 |
| Rmerge | 0.063 * | 0.158 * |
| Total number of observations | 172188 * | |
| Number of reflections | 13617 | |
| <I/σ(I)> | 27.9 | 8.4 |
| Completeness [%] | 99.3 | 94.9 |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | PEG6000, LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | drop | 3 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 17-20 (%(w/v)) | |
| 6 | 1 | reservoir | 1.4-1.7 (M) | ||
| 7 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
