1KNV
Bse634I restriction endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.09932 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 121.230, 122.280, 56.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.610 - 2.170 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25200 * |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.600 | 2.230 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.055 | |
| Number of reflections | 43316 * | |
| Completeness [%] | 95.0 | 87.2 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | Na Acetate, PEG 8000, Calcium chloride, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.10 (mM) | tetramer solution |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.5 |
| 6 | 1 | reservoir | PEG8000 | 12 (%) | |
| 7 | 1 | reservoir | 100 (mM) |
