1KNR
L-aspartate oxidase: R386L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.278, 73.278, 313.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.248 * |
| Rwork | 0.250 |
| R-free | 0.29500 * |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.565 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.107 | 0.373 * |
| Total number of observations | 68503 * | |
| Number of reflections | 26873 | |
| <I/σ(I)> | 4.7 | 1.2 |
| Completeness [%] | 96.1 | 96.4 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 22 * | isopropanol, Hepes, sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES-KOH | 100 (mM) | pH7.5 |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | pH7.5 |
| 3 | 1 | reservoir | 2-propanol | 4-8 (%(v/v)) | |
| 4 | 1 | drop | protein | 50 (mg/ml) | |
| 5 | 1 | drop | HEPES-KOH | 50 (mM) | pH8.0 |
