1KMP
Crystal structure of the Outer Membrane Transporter FecA Complexed with Ferric Citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0003 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 117.467, 88.762, 95.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.950 - 2.500 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.28200 |
| Structure solution method | MAD |
| Starting model (for MR) | Phase information was derived from an additional selenomethionyl derivative |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.169 | |
| Number of reflections | 34980 * | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 98.3 | 0.723 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 284 | Smith, B.S., (1998) Acta Cryst., D54, 697., Doublie, S., (1997) Methods Enzymol., 276, 532. * |
