1KMN
HISTIDYL-TRNA SYNTHETASE COMPLEXED WITH HISTIDINOL AND ATP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 130 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.900, 107.300, 107.200 |
| Unit cell angles | 114.10, 97.40, 90.00 |
Refinement procedure
| Resolution | 13.000 - 2.800 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.34800 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 25.040 * |
| Data reduction software | MARXDS |
| Data scaling software | MARSCALE |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.000 | 2.900 |
| High resolution limit [Å] | 2.600 | 2.800 |
| Rmerge | 0.036 | 0.100 |
| Total number of observations | 116502 * | |
| Number of reflections | 63562 | |
| <I/σ(I)> | 18.7 | 5 |
| Completeness [%] | 84.4 | 82.5 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 20 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | enzyme | 0.07-0.1 (M) | |
| 3 | 1 | drop | histidinol | 50 (mM) | |
| 4 | 1 | drop | ATP | 3 (mM) | |
| 5 | 1 | reservoir | citrate | 0.1 (M) | |
| 6 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | PEG4000 | 30 (%(w/v)) |
