1KM9
The Structure of a Cytotoxic Ribonuclease From the Oocyte of Rana Catesbeiana (Bullfrog)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-25 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.806, 45.918, 57.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.360 - 1.960 |
| R-factor | 0.181 |
| Rwork | 0.178 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rca |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.800 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.360 | 1.980 |
| High resolution limit [Å] | 1.960 | 1.950 |
| Rmerge | 0.055 | 0.196 |
| Number of reflections | 8085 | |
| <I/σ(I)> | 9.4 | 7.06 |
| Completeness [%] | 94.2 | 91.8 |
| Redundancy | 5.62 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 4000, ammonium phosphate, NaOAc, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
