1KM0

Crystal structure of orotidine monophosphate decarboxylase mutant D70N complexed with 6-azaUMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	59.761, 59.869, 73.878
Unit cell angles	97.27, 100.13, 106.93

Refinement procedure

Resolution	30.000 * - 1.700
R-factor	0.179
Rwork	0.179
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	22.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.810
High resolution limit [Å]	1.700	1.700
Rmerge	0.038 *
Total number of observations	452529 *
Number of reflections	96504
Completeness [%]	92.5	67.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	298	Wu, N., (2000) Acta Crystallogr., Sect.D, 56, 912. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	reservoir	trisodium citrate	1.1 (M)	pH6.5
3	1	reservoir	glycerol	3 (%)