1KLO

CRYSTAL STRUCTURE OF THREE CONSECUTIVE LAMININ-TYPE EPIDERMAL GROWTH FACTOR-LIKE (LE) MODULES OF LAMININ GAMMA1 CHAIN HARBORING THE NIDOGEN BINDING SITE

Experimental procedure

Spacegroup name	P 64 2 2
Unit cell lengths	74.570, 74.570, 185.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.1972
Rwork	0.197
RMSD bond length	0.012
RMSD bond angle	25.360 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.070 *
Total number of observations	29004 *
Number of reflections	17012 *
Completeness [%]	91.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.1 (mg/ml)
2	1	drop	Mops	0.62 (mM)
3	1	drop	precipitating buffer		0.0015 ml
4	1	drop	PEG6000	1.0 (%(w/v))
5	1	drop		0.07 (mM)
6	1	reservoir	ammonium sulfate	0.75 (M)
7	1	reservoir	imidazole	0.1 (M)