1KLN
DNA POLYMERASE I KLENOW FRAGMENT (E.C.2.7.7.7) MUTANT/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | AREA DETECTOR |
| Detector | UCSD MARK II |
| Spacegroup name | P 43 |
| Unit cell lengths | 104.300, 104.300, 86.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| Rwork | 0.230 |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 2.700 * |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 17 * | pH 6.80, VAPOR DIFFUSION, temperature 290.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NH4 SULFATE | ||
| 3 | 1 | 1 | CITRATE BUFFER | ||
| 4 | 1 | 1 | MG SULFATE | ||
| 5 | 1 | 1 | ZN SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-12 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 35-40 (%(w/v)sat) | |
| 3 | 1 | reservoir | citrate | 200 (mM) | pH6.8 |
| 4 | 1 | reservoir | 20 (mM) | ||
| 5 | 1 | reservoir | 1 (mM) |
