1KL4
AN ENGINEERED STREPTAVIDIN WITH IMPROVED AFFINITY FOR THE STREP-TAG II PEPTIDE : apo-SAM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2001-07-17 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.450, 86.814, 47.084 |
Unit cell angles | 90.00, 98.82, 90.00 |
Refinement procedure
Resolution | 20.520 - 1.700 |
R-factor | 0.191 * |
Rwork | 0.191 |
R-free | 0.22800 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1swu |
RMSD bond length | 0.018 |
RMSD bond angle | 2.548 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 * | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 * | |
Total number of observations | 178009 * | |
Number of reflections | 48419 | |
<I/σ(I)> | 25.9 | 2.7 |
Completeness [%] | 93.8 | 89.7 |
Redundancy | 3.7 | 3.44 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | 50 mM Na3PO4, 50 mM Na3-citrate, 30% PEG 600, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 22 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |