1KKM
L.casei HprK/P in complex with B.subtilis P-Ser-HPr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 80.832, 80.832, 252.488 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| Rwork | 0.200 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JB1 and 1SPH |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.370 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.040 | 0.146 |
| Total number of observations | 274803 * | |
| Number of reflections | 23597 | |
| <I/σ(I)> | 5.3 | 2.1 |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 3.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | PEG-400, Hepes, CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 28 (%) | |
| 2 | 1 | reservoir | HEPES-Na | 100 (mM) | pH7.5 |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | drop | HprK/P monomer | 0.25 (mM) | |
| 5 | 1 | drop | HPr | 0.5 (mM) | |
| 6 | 1 | drop | PPi | 10 (mM) | presence or absence |
