1KKJ
Crystal Structure of Serine Hydroxymethyltransferase from B.stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.154, 106.637, 56.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.930 |
| Rwork | 0.178 |
| R-free | 0.20340 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dfo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.031 | 0.031 |
| Total number of observations | 68643 * | |
| Number of reflections | 27746 | |
| <I/σ(I)> | 21.8 | 21.8 |
| Completeness [%] | 97.0 | 90.3 |
| Redundancy | 2.47 | 2.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 25 * | Hepes MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 100 (mM) | pH7.5 |
| 2 | 1 | drop | EDTA | 0.2 (mM) | |
| 3 | 1 | drop | 2-mercaptoethanol | 5 (mM) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | drop | protein | 15 (mg/ml) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 7 | 1 | reservoir | EDTA | 0.2 (mM) | |
| 8 | 1 | reservoir | 2-mercaptoethanol | 5 (mM) | |
| 9 | 1 | reservoir | MPD | 50 (%) |
