1KK7
SCALLOP MYOSIN IN THE NEAR RIGOR CONFORMATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 159.849, 51.227, 84.073 |
| Unit cell angles | 90.00, 99.48, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 3.200 |
| R-factor | 0.263 |
| Rwork | 0.259 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dfk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.540 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.109 * | 0.335 * |
| Number of reflections | 20298 * | |
| <I/σ(I)> | 11.2 | 2.4 |
| Completeness [%] | 88.5 | 65.8 |
| Redundancy | 4.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.3 | 4.0 * | PEG 5000, ammonium sulphate, MgCl2, gycerol, cacodylate, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 20 (mM) | pH6.3 |
| 3 | 1 | reservoir | ammonium sulfate | 50.0 (mM) | |
| 4 | 1 | reservoir | 2.0 (mM) | ||
| 5 | 1 | reservoir | glycerol | 8 (%) | |
| 6 | 1 | reservoir | PEG5000 MME | 10 (%(w/v)) |
