1KK0

Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795, 0.9797, 0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	50.871, 86.416, 58.213
Unit cell angles	90.00, 109.75, 90.00

Refinement procedure

Resolution	47.000 - 1.950
R-factor	0.219 *
Rwork	0.219
R-free	0.25200 *
Structure solution method	MIR+MAD
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.040 *	0.219 *
Number of reflections	34217
<I/σ(I)>	11.4	3.3
Completeness [%]	98.9	89.4
Redundancy	3.5	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7 *	297	peg8000, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop		200 (mM)
3	1	drop	MOPS	10 (mM)	pH6.7
4	1	drop	2-mercaptoethanol	10 (mM)
5	1	reservoir	PEG8000	8-18 (%)
6	1	reservoir	Tris-HCl	0.1 (M)	pH8.5