1KJX
IMP Complex of E. Coli Adenylosuccinate Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 120 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-01-01 |
| Detector | SIEMENS |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.600, 80.600, 158.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| Rwork | 0.196 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 20293 | |
| Completeness [%] | 98.0 | 96 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | PEG 8000, HEPES, magnesium acetate, IMP, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
