1KJ8
Crystal Structure of PurT-Encoded Glycinamide Ribonucleotide Transformylase in Complex with Mg-ATP and GAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-06-07 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.100, 179.300, 75.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.22800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1eyz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 16.600 * |
| Data scaling software | SAINT |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.670 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.044 | 0.212 |
| Number of reflections | 108032 | 12764 * |
| <I/σ(I)> | 18.9 | 3.4 |
| Completeness [%] | 97.0 | 92 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.7 | 4 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ATP | 5 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 250 (mM) | ||
| 4 | 1 | drop | protein | 20 (mM) | |
| 5 | 1 | reservoir | PEG5000 ME | 16-24 (%(w/v)) | |
| 6 | 1 | reservoir | MOPS | 100 (mM) | pH6.7 |
| 7 | 1 | reservoir | 100 (mM) |
