1KIF

D-AMINO ACID OXIDASE FROM PIG KIDNEY

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Detector technology	IMAGE PLATE
Collection date	1995-08
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	326.480, 138.100, 200.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.600
R-factor	0.228 *
R-free	0.24500 *
RMSD bond length	0.017
RMSD bond angle	2.800
Data reduction software	MOSFLM
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.600	2.600
Rmerge	0.095
Total number of observations	592028 *
Number of reflections	119327
Completeness [%]	86.0 *	45.2
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.3 *	28 *	293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	sodium benzoate	2 (mM)
3	1	reservoir	ammonium succinate	0.5 (M)
4	1	reservoir	Tris-HCl	100 (mM)	pH8.3