1KIB
cytochrome c6 from Arthrospira maxima: an assembly of 24 subunits in the form of an oblate shell
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 1995-11-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 237.000, 237.000, 237.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.2051 |
| Rwork | 0.204 |
| R-free | 0.22300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f1f |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.450 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.150 * | 0.391 * |
| Number of reflections | 13268 | |
| <I/σ(I)> | 9.3 | 3.4 |
| Completeness [%] | 91.0 | 95.5 |
| Redundancy | 7.1 | 7. * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 298 | 0.1M Tris pH 7.8, 2.4M ammonium sulfate, 5% methylpentanediol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | Tris | 5 (mM) | pH7.5 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH7.8 |
| 5 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
| 6 | 1 | reservoir | MPD | 5 (%) |
