1KHT
Adenylate kinase from Methanococcus voltae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.037 |
Spacegroup name | I 2 3 |
Unit cell lengths | 200.500, 200.500, 200.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.420 - 2.500 |
R-factor | 0.2201 |
Rwork | 0.220 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nks |
RMSD bond length | 0.006 |
RMSD bond angle | 21.800 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.500 | 2.500 * |
Rmerge | 0.066 * | 0.348 * |
Number of reflections | 39038 | |
Completeness [%] | 84.2 * | 86.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 4000, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | AMP | 3 (mM) | |
3 | 1 | drop | ADP | 3 (mM) | |
4 | 1 | reservoir | sodium acetate/acetic acid | 0.1 (M) | pH4.6 |
5 | 1 | reservoir | PEG4000 | 8 (%) |