1KHG

PEPCK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-10-15
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	45.388, 60.872, 61.974
Unit cell angles	88.69, 70.13, 72.37

Refinement procedure

Resolution	20.000 - 2.340
R-factor	0.1901
Rwork	0.185
R-free	0.28073
RMSD bond length	0.041
RMSD bond angle	3.002
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.340
Rmerge	0.066 *
Number of reflections	23934
Completeness [%]	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	289	PEG1500, MES, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	HEPES-KOH	30 (mM)	pH7.5
3	1	drop		2.5 (mM)
4	1	drop	spermidine	1 (mM)
5	1	reservoir	MPD	30 (%(v/v))
6	1	reservoir	sodium citrate	0.1 (M)	pH5.6
7	1	reservoir	ammonium acetate	0.2 (M)