1KHF

PEPCK complex with PEP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-01-08
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	45.251, 60.679, 62.032
Unit cell angles	88.69, 70.00, 72.54

Refinement procedure

Resolution	20.000 - 2.020
R-factor	0.18621
Rwork	0.183
R-free	0.24424
RMSD bond length	0.028
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.020
Rmerge	0.041 *
Number of reflections	34451
Completeness [%]	89.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	289	PEG1500, MES, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	HEPES-KOH	30 (mM)	pH7.5
3	1	drop		2.5 (mM)
4	1	drop	spermidine	1 (mM)
5	1	reservoir	MPD	30 (%(v/v))
6	1	reservoir	sodium citrate	0.1 (M)	pH5.6
7	1	reservoir	ammonium acetate	0.2 (M)