1KHE
PEPCK complex with nonhydrolyzable GTP analog, MAD data
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.971270, 0.978947, 0.978838 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.386, 60.540, 61.833 |
| Unit cell angles | 88.36, 70.39, 72.37 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.17365 |
| Rwork | 0.170 |
| R-free | 0.24572 |
| Structure solution method | MAD |
| RMSD bond length | 0.035 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.020 * |
| Number of reflections | 22522 |
| Completeness [%] | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | 289 | PEG1500, MES, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | HEPES-KOH | 30 (mM) | pH7.5 |
| 3 | 1 | drop | 2.5 (mM) | ||
| 4 | 1 | drop | spermidine | 1 (mM) | |
| 5 | 1 | reservoir | MPD | 30 (%(v/v)) | |
| 6 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 7 | 1 | reservoir | ammonium acetate | 0.2 (M) |
