1KHC

Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2000-05-27
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 65 2 2
Unit cell lengths	52.750, 52.750, 186.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.19 *
Rwork	0.190
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	24.219 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.038	0.148
Total number of observations	145433 *
Number of reflections	15308
<I/σ(I)>	23.6	9.4
Completeness [%]	99.2	94.3
Redundancy	9.5	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	16 *	30% PEG 8000, 0.1M sodium cacodylate, 0.2M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	7 *	16 *	30% PEG 8000, 0.1M sodium cacodylate, 0.2M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	drop	HEPES	20 (mM)	pH7.0
3	1	drop		200 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop	glycerol	5 (%(v/v))
6	1	reservoir	PEG8000	30 (%(w/v))
7	1	reservoir	sodium acetate	200 (mM)
8	1	reservoir	sodium cacodylate	100 (mM)	pH6.5