1KHB
PEPCK complex with nonhydrolyzable GTP analog, native data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.400, 60.580, 62.000 |
| Unit cell angles | 88.40, 70.30, 72.63 |
Refinement procedure
| Resolution | 20.000 - 1.854 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22473 |
| RMSD bond length | 0.022 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.854 |
| Rmerge | 0.027 * |
| Number of reflections | 47525 |
| Completeness [%] | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 289 | PEG1500, MES, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 2 | 1 | drop | protein | 5 (mg/ml) | |
| 3 | 1 | drop | HEPES-KOH | 30 (mM) | pH7.5 |
| 4 | 1 | drop | 2.5 (mM) | ||
| 5 | 1 | drop | spermidine | 1 (mM) | |
| 6 | 1 | reservoir | MPD | 30 (%(v/v)) | |
| 7 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 8 | 1 | reservoir | ammonium acetate | 0.2 (M) |
